• Bio-NEST Equiptment

    Bio-NEST offers equipment and instrumentation to support biotechnology research, development, and commercialization activities.

    Printable List in PDF Format

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    Thermal Cycler (PCR)

    Make: Bio-rad
    Model: T100
    Sample Capacity: 96 x 0.2ml tubes, 0.2 ml tube strips, or 1 x 96-well plate
    Maximum Ramp Rate: 4°C/sec
    Temperature Range: 4°C-100°C
    Gradient Range: 30°C-100°C
    Temperature Differential Range: 1°C-25°C

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    Electroporator

    Make: Bio-rad
    Model: Gene Pulser XcellTM
    Waveform: Exponential or square
    Capacitance: 10–500 V: 25–3,275 µF in 25 µF increments; 500–3,000 V: 10, 25, 50 µF
    Resistance (parallel): 50–1,000 Ω in 50 Ω increments, plus infinity
    Sample Resistance: 20 Ω minimum at 10–2,500 V; 600 Ω minimum at 2,500–3,000 V

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    Microplate Reader

    Make: PerkinElmer

    Model: VictorⓇ NivoTM S

    Modes: Absorbance (spectrometer), Fluorescence and Luminescence

    Filters: 405/10, 480/30, 530/30, 700/IR Blocker, Unfiltered LUM,

    TRF Filters: 320/75, 615/8, 355/40, 435/20, 460/30, 495/20, 540/30, 580/20, 625/30, 640/30, 685/30

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    Horizontal Gel Electrophoresis

    Make: Bio-rad
    Model: Mini-Sub Cell GT
    UV-transparent Tray: 7x10cm, 15x15cm
    Comb: 15- and 20-well
    Casting System: Yes

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    Vertical Gel Electrophoresis

    Make: Bio-rad
    Model: Mini-Protean® Tetra System
    Comb: 10-well
    Gel Capacity: Maximum 4 at a time
    Precast: 8.6 x 6.8 cm; handcast: 8.3 x7.3 cm
    Plates: with 1mm integrated spacer

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    Gel-Doc

    Make: Bio-rad
    Model: ChemiDoc™ Imaging System High-sensitivity imaging of DNA/protein gels and chemiluminescent western blots Data transfer through LAN and USB interface Pre-defined protocols Auto exposure

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    Lyophilizer

    Make: Lyophilization Systems
    Model: Lyolab 3S
    Shelf Temperature: -60°C to +65°C
    No. of shelves: 3
    Total Shelf Area: 3.75 sq ft
    Vacuum: 10mT
    Cycles: Freeze dry, Defrost, Semi-Auto Fully Programmable Software with real-time monitoring and data logging, recipe management, protocol transfer and data report 3 probes for sample temperature monitoring; Nitrogen backfill and vial stoppering available

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    Autoclave

    Make: Equitron
    Model: 7441FA
    Capacity: 110 L
    Digital control unit Fully programmable with cycle time and temperature control Low water level alarm Stale air purging and auto-release of steam

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    High-speed Refrigerated Centrifuge

    Make: Thermofisher Scientific
    Model: Sorvall Legend X1R
    Speed: 300-15200 rpm (max 25830 x g)
    Temperature Range: -10°C to +40°C
    Rotors: Swinging bucket with 36 x 15ml/ 16 x 50ml/ 4 x 400ml capacity fixed-angle with 8 x 15ml/ 8 x 50ml/ 30 x 2ml capacity

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    Probe Sonicator

    Model: Q500
    Make: Qsonica
    Pulse On/Off: 1 sec to 1 min
    Total Programme Time: 10 hrs
    Frequency: 20 kHz
    Probes: Part no. Diameter Processing volume #4220 12.7 mm 20-250 ml #4418 3.2 mm 1-15 ml #4420 6.4 mm 5-50 ml #4209 25.4 mm 100-1000 ml

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    Shaker Incubator

    Model: ORBITEK® - LE4676
    Make: Scigenics Biotech Private Limited
    Temperature Range: 5°C to 80°C
    Speed Range: 25-400 RPM
    Humidity Display: 0-100%
    Modes: Programme, Continuous
    Data Logging: Yes, USB drive
    Dimensions: (Internal) 850 x 612 x 453 mm
    Flask Holding Capacity: 100ml Erlynmeyer flask (4), 250ml Erlynmeyer flask (8), 500ml Erlynmeyer flask (5), 1000ml Erlynmeyer flask (5), 2000ml Erlynmeyer flask (5), 5000ml Erlynmeyer flask (3)

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    Fluorescent Cell Imager

    Model: Zoe™
    Make: Bio-Rad
    Multi-channel merging (upto 4) Touch screen Interface Brightfield and three fluorescent channels
    Blue Ex: 355/40 nm Em: 433/36 nm
    Green Ex: 480/17 nm Em: 517/23 nm
    Red Ex: 556/20 nm Em: 615/61 nm
    Compatible with Flasks: T25, T75, or T225
    Multiwell Plates: 6-, 12-, 24-, 48-, 96-, or 384-well microplates
    Dishes: 35 mm, 60 mm, or 100 mm
    Slides: Chamber slides or standard glass microscopy slides

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    CO2 Incubator

    Model: FormaTM Steri-cycle TM i160
    Make: Thermo Fisher Scientific
    Capacity: 165 L
    Relative humidity: <93% at 37°C
    CO2 Concentration Range: 1 to 20%
    CO2 Sensor Technology: IR Sensor
    Temperature Range: Ambient +3°C to 55°C

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    Walk-in cold chamber

    Model: Custom make
    Make: Swastik Enterprises
    Area: ~140 sq. ft.
    Temperature Range: 4°C to ambient temp.
    2 x work benches; 1 x shelf rack 1 x wash basin; 4 x power points

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    HPLC

    Model: Nexera Prep
    Make: Shimadzu
    Columns: C18, 5µm, 4.6mm x 250mm; C18, 5µm, 20mm x 250mm

    Pump: Binary, 0.01-150ml/min

    Sample loop size: 20µl, 500µl, 2ml, 5ml

    Fraction collector: Yes

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    FPLC

    Model: Akta Pure 25 M1

    Make: Cytiva

    Flow rate: 0.001 to 25ml/min (normal range), 0.01 to 50ml/min (sample pump)

    Injection modes: loop, direct, sample pump Detectors: multi-wavelength UV, pH, Conductivity

    Operating pressure: <20 MPa

    Fraction Collector: Yes

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    Biosafety Cabinet

    Model: AC2-2E8

    Make: ESCO Biotech

    Internal Dimensions (W x D x H) (in mm): (610 x 580 x 660)

    ULPA Filter Efficiency: >99.99% at 0.1 to 0.3 micron

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    Gas Chromatograph

    Model: GC-2014c

    Make: Shimadzu

    Column temperature: (Room temperature +10°C) to 400°C

    Carrier gas control: Digital setting by electronic flow controller (AFC)

    Sample injector: Dual for packed, single for packed,

  • Bio-NEST Usage Policy Snapshot

    • Facility timings are from 9:00 am to 6:00 pm. The facility will be closed on Saturdays, Sundays and other public holidays as per the Ahmedabad University Calendar.
    • All instruments need to be booked before usage, either offline through a log sheet or online through the booking portal. Usage charges will be levied as per the rate card.
    • Access to any instrument is subject to its availability. Users must check instrument availability, functionality, and booking and cancellation terms before booking any instrument.
    • Users will be allowed to handle instruments only after undergoing basic training. Usage of certain instruments may only be allowed under supervision. PPE such as a lab coat, gloves, safety goggles or other items required must be brought in by the user.
    • Users are required to bring their own consumables. Generic items like plasticware and chemicals can be provided based on availability at extra charges as applicable.

    Bio-NEST Rate Card

    • Basic Instrument and Wet Lab Package - Rs. 7200/month (Rs. 500/day)
    • Above package includes workspace for 2 team members with storage, basic instrumentation and microbiology lab
    • Add-on packs for Cell culture@ Rs. 4800/month and Molecular biology@Rs. 3600/month
    • Billing will be processed at the beginning of each month, on a monthly basis. We do not have a GST number.
  • Equipment Inquiry Form

    Please fill out this form, and we will get back to you within 48 hours.